• I am in G3.
    • Self evaluation May 19 (G3,G4,G6)
    • If you pass, no need for histology evaluation (the abilitante pre-written exam one)

Schedule

  1. Covering Epithelia
  2. Glandular Epithelia - Exocrine
  3. Glandular Epithelia - Endocrine
  4. Connective Tissue Proper - Hematopoietic system
  5. Connective Tissue - Bone and Cartilage
  6. Nerve Tissue and Muscle Tissue
  7. Summary- Rehearsal/Recap

Syllabus

  • Introduction to the histotechnology by detailing tissue preparation procedures, techniques for essential histological identifications by light microscope, commons staining and relevant protocols for cell shape and size identification: 1h.
  • Covering Epithelia
    • Distinguish between, simple, stratified and pseudostratified epithelia
    • Cell shape characteristic of squamous, cuboidal, columnar and transitional epithelia
    • Identification of surface specializations of epithelia
    • Endocrine and exocrine epithelia
    • Organization and function of exocrine tissues.
    • The modality of secretion (apocrine, exocrine, merocrine and holocrine secretion)
    • Organization and function of endocrine tissue
  • Connective Tissues
    • Identification of the structural organization both of fibers in the extracellular matrix (ECM) and the cells residing within connective tissue
    • Loose and dense connective tissue using the light microscope
    • White and brown adipose tissue
    • Cartilage classification (hyaline, fibrous and elastic)
    • Bone tissue, Intramembranous and Endochondral ossification
    • Observe and describe the processes of bone remodeling and bone growth.
    • Describe the calcification processes of cartilage and bone.
  • Blood
    • Identify the component cells of a blood smear
    • Identify the hematopoietic precursor cells of a typical bone marrow smear
    • Distinguish between the different classes of white blood cells
  • Muscle Tissue and Nervous System
    • Identify the histological landmarks of skeletal muscle
    • Contrast the structure and function of skeletal, smooth, and cardiac muscle tissue.
    • Identify morphological differences in smooth muscle across tissues.
    • Explain the structure and function of the intercalated disc.
  • Histological and structural features of neurons
    • Microscopic structure of a nerve fiber, including the structure of the myelin sheath and connective tissue layers.
    • Identify the four types of glial cells, their structures, and their functions.
  • Hands on training on two different technics of tissue staining. 2h
    • hematoxylin and eosin of unknown specimens of murine derivation and immunofluorescence staining of cytoskeleton components, namely actin filaments (also known as F-actin).